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All functions

build_se()
Build a SummarizedExperiment from transcript readcounts and tx->gene map
calculate_difference()
Calculate splicing diversity changes between two conditions.
calculate_diversity()
Calculate Tsallis diversity per gene across samples
calculate_fc()
Calculate splicing diversity changes between two conditions.
calculate_lm_interaction()
Linear-model interaction test for Tsallis entropy For each gene, fit a linear model of the form `entropy ~ q * group` and extract the p-value for the interaction term (whether the effect of `q` differs between groups). The function expects a `SummarizedExperiment` produced by `calculate_diversity()` when multiple `q` values have been computed (column names contain `_q=`).
calculate_method()
Calculate Tsallis diversity values for transcripts grouped by gene
calculate_tsallis_entropy()
Calculate Tsallis entropy for a vector of transcript-level expression values of one gene.
extract_tx2gene_from_gff3()
Extract Transcript-to-Gene Mapping from GFF3 File
filter_se()
Filter transcripts in a `SummarizedExperiment` by minimum count/sample
label_shuffling()
Calculate p-values using label shuffling.
map_metadata()
Map external coldata into a SummarizedExperiment
map_tx_to_readcounts()
Map transcript IDs from a tx2gene table to a readcounts matrix
plot_diversity_density()
Plot diversity distributions (density) by sample type
plot_ma_expression()
Plot MA using expression/readcount-based fold changes
plot_ma_tsallis()
Plot MA using Tsallis-based fold changes
plot_mean_violin()
Plot violin of per-gene mean diversity by sample type
plot_top_transcripts()
Plot top transcripts for a gene
plot_tsallis_density_multq()
Density plot of Tsallis entropy for multiple q values
plot_tsallis_gene_profile()
Plot q-curve profile for a single gene comparing groups
plot_tsallis_q_curve()
Plot median +- IQR of Tsallis entropy across q values by group
plot_tsallis_violin_multq()
Violin plot of Tsallis entropy for multiple q values
plot_volcano()
Volcano plot for differential results
tcga_brca_luma
TCGA Luminal A breast cancer dataset (transcript-level)
test_differential()
Run a differential test by name: Wilcoxon or label-shuffle
wilcoxon()
Calculate p-values using Wilcoxon rank sum test.